NAD+ (Nicotinamide Adenine Dinucleotide)
Certificate of Analysis (COA) data provided in product photos.
Purity: ≥ 99 % (HPLC verified)
Sterility: Confirmed (sterile-filtered)
Endotoxins (LPS): Not Detected
Trifluoroacetic Acid (TFA): Not Detected (LC-MS verified)
Identity: Confirmed (LC-MS verified)
Quantity: Verified (HPLC analysis)
Format: Lyophilized powder sealed in an airtight vial to maintain long-term stability during storage.
Pricing
| Quantity | Price/Vial | Savings |
|---|---|---|
| 1 vial | $150 | — |
| 2 vials | $144 | Save 4 % |
| 3 vials | $138 | Save 8 % |
| 4 vials | $132 | Save 12 % |
| 5 vials | $126 | Save 16 % |
Product Overview
NAD+ (Nicotinamide Adenine Dinucleotide) is a ubiquitous coenzyme present in every living cell and represents the biologically active form of vitamin B3 (niacin). It serves as an essential electron carrier in metabolic redox reactions and as a substrate for key enzyme families involved in DNA repair, epigenetic regulation, and cellular stress responses.
Molecular Formula: C₂₁H₂₇N₇O₁₄P₂
Molecular Weight: 663.4 g/mol
PubChem CID: 5892
Alternate Names: nadide, coenzyme I, β-NAD
Biological Background (For Research Context Only)
Core Biochemical Functions
In laboratory and biochemical studies, NAD+ participates in two fundamental cellular processes:
Energy Metabolism (Redox Cofactor)
Transfers electrons during glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation.
Alternates between oxidized (NAD⁺) and reduced (NADH) forms to support ATP generation.
Enzymatic Regulation (Non-Redox Substrate)
Serves as a substrate for enzymes such as sirtuins (SIRTs) and poly(ADP-ribose) polymerases (PARPs), which link cellular energy status to DNA repair, chromatin structure, and stress-response pathways.
Acts as a precursor for ADP-ribosylation reactions and second-messenger molecules (e.g., cADPR) studied in calcium signaling.
Cellular Processes Associated with NAD+ Activity
Research has investigated NAD+ in relation to several major cellular systems:
Genomic Maintenance – Functions as a cofactor for DNA-repair enzymes and poly(ADP-ribosyl) reactions.
Epigenetic Regulation – Sirtuin-dependent deacetylases utilize NAD+ to influence chromatin structure and gene expression.
Mitochondrial Function – Essential for electron-transport-chain activity and oxidative energy production.
Autophagy & Proteostasis – Supports cellular recycling and protein-quality control pathways under metabolic stress.
Redox Homeostasis – Maintains cellular NAD⁺/NADH balance important for metabolic equilibrium.
Metabolic Turnover and Regulation
The cellular NAD+ pool is continuously consumed and recycled through metabolic pathways including the salvage cycle.
NAMPT (nicotinamide phosphoribosyltransferase) is a key enzyme in NAD+ biosynthesis from nicotinamide.
CD38 and PARP enzymes consume NAD+ during signaling and DNA repair.
Preclinical research suggests that changes in these pathways can influence overall cellular energy status and redox balance.
Research Applications and Context
NAD+ is widely used in cellular metabolism, aging, and neurobiology research to study how cells sense and respond to energy demand, oxidative stress, and environmental conditions.
Investigations often focus on:
Mitochondrial biogenesis and quality control in energy-intensive tissues.
Sirtuin-mediated stress responses and genomic stability mechanisms.
PARP-dependent DNA repair dynamics following oxidative damage.
Metabolic homeostasis and the balance between NAD+ consumption and recycling across organ systems.
These studies contribute to a deeper understanding of how NAD+ connects bioenergetic status with cellular adaptation pathways.
Storage & Handling
Store at 4 °C in a dry, light-protected environment for medium-term storage. Reconstitute with sterile, bacteriostatic solvent immediately before use. Proper storage maintains peptide integrity. Lyophilized NAD+ can be stored at 4 °C for months, while reconstituted NAD+ needs to be used within 7-10 days while stored at 4 °C.
Intended Use and Compliance
All products from Peptide Systems are intended for laboratory and scientific research use only.
This product is not FDA-approved and not intended for human or veterinary consumption.
Purchase requires acknowledgment and acceptance of our Terms and Conditions.
References & Further Reading
NAD+ Metabolism and Signaling - https://www.cell.com/cell-metabolism/fulltext/S1550-4131(19)30316-X
Sirtuins and NAD+ in Metabolic and Cardiovascular Diseases - https://pmc.ncbi.nlm.nih.gov/articles/PMC6206880/
NAD+ Synthesis and Functions - https://pubmed.ncbi.nlm.nih.gov/32595066/
Age Related Changes in NAD+ Metabolism - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0019194
NAD+
Buy 5 vials get 16% off
CERTIFIED by 3rd party, US registered labs with ISO 17025:2017 certification
- Certified sterile.
- Certified free of microbial contaminants (endotoxin, LPS, LAL).
- Certified free of chemical contaminants (TFA, IEX).
- Certified peptide identity (HPLC, LC-MS).
- Certified >99% pure (HPLC).
